molecular identification and capsular typing of pasteurella multocida isolates from sheep pneumonia in iran
نویسندگان
چکیده
pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. as immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. the aim of this study was to molecular identification and determine of the capsular type of the p. multocida strains isolated from sheep pneumonia in iran. bacteriological and biochemical characterization was confirmed by species specific pm-pcr. genomic dna was extracted by boiling method. capsular typing was conducted by using type specific primers via cap-pcr method. the pcr product was sequenced and analysed by blast software. the biochemical identification was confirmed by a species-specific pcr assay (pm-pcr). according to cap-pcr results, all of the 52 p.multocida isolates belonged to the capsular type a. the pcr amplified a fragment of 1044 bp from all of tested isolates. the sequence alignment of the cap-pcr product showed a high similarity (>98%) with the published sequences of p.multocida hya gene in the gene bank. it was found that capsular type a is dominant among p. multocida isolates from sheep pasteurellosis in endemic areas of iran. investigation on preparation and evaluation of an effective sheep pasteurellosis vaccine by using ovine isolates of p.multocida capsular type a is recommended.
منابع مشابه
Molecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran
Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and determine of the capsular type of the P. multocida strains isolated from sheep pneumonia in Iran. Bacteriological and bio...
متن کاملMolecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran
ARTICLE INFO ABSTRACT Article history: Received: 2 May 2014 Accepted: 12 August 2014 Available online: 1 December 2014 Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and de...
متن کاملMolecular and Clinical Microbiology Molecular identification and capsular typing of Pasteurella multocida isolates from sheep pneumonia in Iran
ARTICLE INFO ABSTRACT Article history: Received: 2 May 2014 Accepted: 12 August 2014 Available online: 1 December 2014 Pasteurella multocida is known as one of the main organisms causing pneumonia in sheep. As immunity in pasteurellosis is serogroup specific, identification of prevalent capsular group among endemic areas is essential. The aim of this study was to molecular identification and de...
متن کاملCharacterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats
Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA gen...
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عنوان ژورنال:
international journal of molecular and clinical microbiologyجلد ۴، شماره ۲، صفحات ۴۱۷-۴۲۳
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